How do I read my Enterotube results?

The presence of acetoin, a positive result, is indicated by the development of a red color within 20 minutes, a negative reaction will appear colorless or light amber. The use of Barritt’s reagent in the VP chamber will be performed after all the results are read for the other chambers in the enterotube.

What does Enterotube II test for?

The Enterotube II is a compartmental plastic tube containing 12 media to test for: acid and gas production from glucose, lysine decarboxylase, ornithine decarbox- ylase, H2S, indole, acetoin (VP test), phenylalanine de- aminase, urease, citrate utilization, and acid production from adonitol, lactose, arabinose.

Which group of bacteria is identified by Enterotube?

family Enterobacteriaceae
The Enterotube™ II is an example of a rapid, multi test system used in identification of unknown oxidase- negative, gram- negative, rod shaped bacteria of the family Enterobacteriaceae.

How do you test for Pseudomonas aeruginosa?

Detection of P. aeruginosa colonisation is normally achieved by culture of wound swabbing on to artificial media. Typical isolation media for wound infections include blood agar and chocolate agar as well as selective agars such as Mac- Conkey agar and cetrimide-based media.

How does EnteroPluri-test work?

EnteroPluri-Test is a 12-sector system containing special culture media that permits identification of the Enterobacteriaceae and other gram negative, oxidase negative bacteria. The system allows the simultaneous inoculation of all media present in the sectors and the execution of 15 biochemical reactions.

What might be the benefit of using an Enterotube?

It was concluded that the Enterotube system provides a simple, reliable, and rapid method for the presumptive identification of Enterobacteriaceae. The major advantage of the Enterotube is that all tests are done simultaneously by inoculation from a single isolated colony.

What is API testing in microbiology?

The analytical profile index or API is a classification of bacteria based on biochemical tests, allowing fast identification. This system is developed for quick identification of clinically relevant bacteria. A bacterial suspension is used to rehydrate each of the wells and the strips are incubated.

How do I use API 20e?

Add sterile oil into the ADH, LDC, ODC, H2S and URE compartments. Put some drops of water in the tray and put the API Test strip and close the tray. Mark the tray with identification number (Patient ID or Organism ID), date and your initials. Incubate the tray at 37oC for 18 to 24 hours.

How do I read my EnteroPluri-test results?

Indole test Lay EnteroPluri-Test with its flat surface pointing upward and, by punching the plastic film, add 3 or 4 drops of Kovac’s Indole Reagent in the sector H2S/Indole. The reaction is positive if a pink-red colour develops in the added reagent within 10-15 seconds.

Why does Pseudomonas aeruginosa grow in the ear?

Pseudomonas aeruginosa loves to grow in warm moist aerobic environments, so the conditions in the external auditory meatus are perfect for it. These bacteria colonise the ear so when samples are sent to the lab, they grow easily.

What do you need to know about the Enterotube II?

The Enterotube™ II consists of a tube with a flat side and contains 12 compartments for different biochemical tests. It has prepared sterile multimedia tube for rapid differential identification of Gram-negative bacteria (Enterobacteriaceae).

How to reinsert wire into BBL Enterotube II?

Reinsert wire (without sterilizing) into BBL Enterotube II, using a turning motion through all 12 compartments, until the notch on the wire is aligned with the opening of the tube. The tip of the wire should be seen in the citrate compartment. Break wire at notch by bending.

How long do you incubate BBL Enterotube II?

Incubate at 35 to 37° C for 18 to 24 hours with BBL Enterotube II lying on its flat surface or in an upright position. Allow for air circulation between incubated tubes.

How do I read my Enterotube results? The presence of acetoin, a positive result, is indicated by the development of a red color within 20 minutes, a negative reaction will appear colorless or light amber. The use of Barritt’s reagent in the VP chamber will be performed after all the results are read for the…