How do you make carbol Fuchsin?

Carbol fuchsin (1%) was prepared from 10 g of basic fuchsin (Hi-Media) dissolved in 100 ml of methanol (Qualigens) and 50 ml of melted phenol (Qualigens) in a flask maintained at 60°C in a water bath. This solution was made up to 1,000 ml with distilled water.

What is the difference between Ziehl-Neelsen and kinyoun stain?

The fundamental difference between the two carbolfuchsin-based methods is whether heat is used during the primary staining process. The Ziehl-Neelsen method uses heat to infuse the carbolfuchsin into the acid-fast cells, whereas the Kinyoun method does not use heat.

What is the wetting agent used in kinyoun method?

The wetting agent, tergitol, reduces surface tension between the cell wall of the mycobacteria and the stain allowing it to penetrate.

Is carbol fuchsin acidic or basic?

Carbol Fuchsin is a basic dye which forms a yellow-brown compound in presence of acid. It is mainly required for the staining of mycobacteria. The waxy walls of mycobacteria helps in retaining the dye within cells. It can also be used for the staining of bacterial spores.

What color is basic fuchsin?

Dark green
Basic fuchsin staining is certified to use for the detection of acid-fast bacilli and is commonly used in the Ziehl Neelsen staining method. This dye has also been shown to stain tubercle Bacillus and to compare E. coli and Aerobacter aerogenes. Color: Dark green powder (will turn red in solution).

Why ZN staining is called acid-fast?

Acidfast Stain: Background and Introduction. Mycobacterium and many Nocardia species are called acid-fast because during an acid-fast staining procedure they retain the primary dye carbol fuchsin despite decolorization with the powerful solvent acid-alcohol.

Is Safranin acidic or basic?

Safranin is a basic biological dye commonly used as a counter-stain in some of the staining protocols like gram staining.

What Counterstain is used in kinyoun method?

methylene blue
It involves the application of a primary stain (basic fuchsin), a decolorizer (acid-alcohol), and a counterstain (methylene blue). Unlike the Ziehl–Neelsen stain (Z-N stain), the Kinyoun method of staining does not require heating.

Why it is called acid-fast?

Once stained as part of a sample, these organisms can resist the acid and/or ethanol-based decolorization procedures common in many staining protocols, hence the name acid-fast.

Is basic fuchsin and carbol Fuchsin same?

Carbol fuchsin, carbol-fuchsin, or carbolfuchsin, is a mixture of phenol and basic fuchsin, used in bacterial staining procedures. Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells wall lipids than in the acid alcohol.

How do you make carbol Fuchsin? Carbol fuchsin (1%) was prepared from 10 g of basic fuchsin (Hi-Media) dissolved in 100 ml of methanol (Qualigens) and 50 ml of melted phenol (Qualigens) in a flask maintained at 60°C in a water bath. This solution was made up to 1,000 ml with distilled water. What is…