How do you use RNase Zap?
How do you use RNase Zap?
Apply RNaseZap Solution liberally to a paper towel and wipe all exposed surfaces of the apparatus thoroughly. Rinse with water and then wipe dry. Some small parts may be cleaned by briefly soaking them in RNaseZap Solution, rinsing them with water and then drying.
How long does RNase ZAP last?
Storage and Stability: Store RNaseZap at room temperature. RNaseZap is stable for 6 months from the date received. Safety: If contact with eyes occurs, immediately flush with water and call physician.
Does RNase zap remove DNA?
RNaseZap® RNase Decontamination Solution is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap® Solution onto the surface to be decontaminated and rinse it off with RNase-free water.
How do you remove RNA from surfaces?
Maintain a separate area for RNA work. Carefully clean the surfaces. Decontaminate glassware by baking at 180°C or higher for several hours or by soaking in freshly prepared 0.1% (v/v) DEPC in water or ethanol for 1 hour, followed by draining and autoclaving.
What is the role of RNase?
Ribonucleases (RNases) are a large group of hydrolytic enzymes that degrade ribonucleic acid (RNA) molecules. These are nucleases that catalyze the breakdown of RNA into smaller components. They are a superfamily of enzymes which catalyze the degradation of RNA, operating at the levels of transcription and translation.
How do you remove RNase or dnase from plastic containers?
Soak in a 0.1% aqueous solution of diethyl pyrocarbonate2 (DEPC) for 2 hours at 37°C (99°F); rinse several times with sterile (DEPC-treated) water*; heat to 100°C (212°F) for 15 minutes or autoclave for 15 minutes at 121°C (250°F) on a liquid/slow exhaust cycle. (Heating or autoclaving will remove DEPC residues).
Does bleach deactivate RNase?
It turns out, the active component of bleach, sodium hypochlorite (approx. 6%), can denature proteins through oxidation. Now, this property has been put to good use against RNAses by incorporating a small amount of bleach into the agarose gel before melting and casting.
What does RNase away do?
RNase AWAY® Reagent is a ready-to-use solution for eliminating RNase contamination from labware. Apply it evenly over the surface of glassware or plasticware to be treated and then rinse it away with distilled water. Unwanted RNase contamination are eliminated.
Does RNase away degrade RNA?
The protein concentration of RNase, DNase-free is 0.5 μg/μL The specific activity of the enzyme is 30 U/mg, corresponding to 1.5 mU/μL; one microliter of the RNase preparation is sufficient to completely degrade 15 μg RNA in 30 min.
Does autoclaving get rid of RNase?
Merely autoclaving will not destroy all RNase activity, since these enzymes are very robust and can regain partial activity upon cooling to room temperature. Always use tips and tubes that have been tested and certified RNase-free.
How do you ensure RNase-free environment?
Tips for Maintaining an RNase-free Environment. When working with RNA, wear gloves at all times. After putting on gloves, avoid touching contaminated surfaces and equipment with the gloved hands.
How is RNase Zap used for surface decontamination?
See alternate available products . RNaseZap™ RNase Decontamination Solution is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap™ Solution onto the surface to be decontaminated and rinse it off with RNase-free water.
How is rnasezap used to remove RNase from glass?
General description RNaseZAP™ is a purifying agent used for eliminating RNase contamination from glassware, plastic surfaces, reaction vessels, countertops and pipettors. It is also used for effective removal of RNase residues from microcentrifuge tubes without inhibiting subsequent enzymatic reactions.
Why do we need a RNAse free environment?
Working with RNA requires that special measures be taken to ensure an RNase-free environment. Even trace quantities of RNase can lead to lower yields from in vitro transcription reactions, degradation during RNA purification protocols, and variable results in RPAs and Northerns.
How many primers are in a RNA panel?
The RNA panel is provided in one pack of 8 tubes (Myeloid RNA Pool 1). Each primer pool in the panel is provided in pairs of tubes, where each tube pair contains one tube with primers in position 1 and one empty uncapped tube in position 2.
How do you use RNase Zap? Apply RNaseZap Solution liberally to a paper towel and wipe all exposed surfaces of the apparatus thoroughly. Rinse with water and then wipe dry. Some small parts may be cleaned by briefly soaking them in RNaseZap Solution, rinsing them with water and then drying. How long does RNase ZAP…