What is CT 26 cell line?

CT26. WT (ATCC® CRL-2638™) is a murine colorectal carcinoma cell line from a BALB/c mouse. It is a clone of the N-nitroso-N-methylrethane-induced undifferentiated CT26 colon carcinoma cell line. The cells are adherent and have a fibroblast morphology.

What are EMT6 cells?

EMT6 (ATCC® CRL-2755™) is a murine mammary carcinoma cell line derived from a transplanted hyperplastic alveolar nodule of a BALB/c mouse1. In syngeneic mice, EMT6 cells form tumors and spontaneous metastases, primarily to the lungs2,3.

What is a syngeneic mouse model?

What is a syngeneic mouse model? Syngeneic mouse models, also known as Allograft mouse tumor systems, consist of tumor tissues derived from the same genetic background as a given mouse strain. As the syngeneic mice retain intact immune systems, they are particularly relevant for studies of immunotherapies.

What is B16F10?

B16F10 (ATCC® CCL-6475™) is a murine melanoma cell line from a C57BL/6J mouse. It is a subclone of the B16 tumor line, generated by injecting mice with B16 tumor cells, collecting and culturing secondary tumor growths, and injecting them into fresh mice, a total of 10 times.

Why are SCID mice used?

SCID mice, which virtually lack both T and B lymphocytes and accept xenogeneic cells, have been used during the last 5 years to provide a milieu for lymphocytes isolated from individuals with various autoimmune diseases, or for lymphocytes from mice that have a systemic lupus erythematosus-like syndrome.

What kind of cell line is ct26.wt?

CT26 is an N-nitroso-N-methylurethane- (NNMU) induced, undifferentiated colon carcinoma cell line. It was cloned to generate the cell line designated CT26.WT ( ATCC CRL-2638 ). Yes, in BALB/c mice.

Is the CT26 colon carcinoma cell line a clone?

The cell is a clone of the N-nitroso-N-methylrethane-induced undifferentiated CT26 colon carcinoma cell line. These cells are adherent and have a fibroblast morphology.

Are there any CT26 cells in BALB / c mice?

CT26.WT-iRFP-Neo is a polyclonal population of the mouse colorectal carcinoma cell line CT26.WT. Yes, in BALB/c mice. Mice are inoculated, subcutaneously, developed lethal tumors at 80% frequency with 10 3 cells and at 100% with 10 4 cells. Pulmonary metastases develop when mice are inoculated, intravenously, with 10 4 cells.

What should the growth medium be for CT26 cells?

Cells that are difficult to detach can be placed at 37°C to facilitate dispersal. Add 6.0-8.0 mL of complete growth medium and then aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to the new culture vessels.

What is CT 26 cell line? CT26. WT (ATCC® CRL-2638™) is a murine colorectal carcinoma cell line from a BALB/c mouse. It is a clone of the N-nitroso-N-methylrethane-induced undifferentiated CT26 colon carcinoma cell line. The cells are adherent and have a fibroblast morphology. What are EMT6 cells? EMT6 (ATCC® CRL-2755™) is a murine mammary carcinoma…