What is pGL3 Basic?
What is pGL3 Basic?
www.promega.com 1. Description. The pGL3 Luciferase Reporter Vectors(a) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors.
Is luciferase a plasmid?
Firefly and Renilla luciferase are less-than-ideal protein tags due to their large size. Luciferase-containing plasmids are commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression.
Who named luciferin?
He discovered that the juice contained two substances which, when mixed, were responsible for the glow. One he named luciferin after Lucifer, the light bearer, and the other he called luciferase to indicate that it had the properties of an enzyme. to isolate luciferin, establish its structure, and synthesize it.
How is luciferase measured?
Luciferase activity is measured using a Luminometer equipped with an automated dual injection system. A volume of 20 μl of cell lysate is transferred to a microcentrifuge tube and placed in the injection chamber.
Do human bodies glow?
The human body literally glows, emitting a visible light in extremely small quantities at levels that rise and fall with the day, scientists reveal. Past research has shown that the body emits visible light, 1,000 times less intense than the levels to which our naked eyes are sensitive. …
How to analyze a plasmid in PGL3 basic?
Plasmid: pGL3-Basic Source/Vendor: Promega Analyze: Sequence Plasmid Type: Luciferase Cloning Method: Unknown Size: 4818
Which is the promoterless vector for luciferase assay?
pGL3-Basic. Promoterless vector for measuring the activity of promoter and enhancer sequences with a luciferase assay. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer.
What are the PGL3 luciferase reporter vectors designed for?
The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly ( Photinus pyralis ) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells.
How to measure promoter and enhancer sequences in snapgene?
Promoterless vector for measuring the activity of promoter and enhancer sequences with a luciferase assay. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer.
What is pGL3 Basic? www.promega.com 1. Description. The pGL3 Luciferase Reporter Vectors(a) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. Is luciferase a plasmid? Firefly and Renilla luciferase are less-than-ideal protein…