How do you test for mitochondrial function?

Traditionally, mitochondrial function has been assessed with Clark-type electrode probes for measuring oxygen consumption, luminescent ATP assays for quantification of total energy metabolism, and MTT or Alamar Blue for determination of metabolic activity.

How is mitochondrial damage measured?

A very common approach to address mitochondrial bioenergetic dysfunction is to measure the expression, concentration or maximum activity of a few candidate electron transport complexes or metabolic enzymes, such as complex I, complex IV or tricarboxylic acid-cycle enzymes.

What is mitochondrial toxicity?

Mitochondrial toxicity is a condition in which the mitochondria of a body’s cells become damaged or decline significantly in number; it occurs as a side effect of certain antiretroviral drugs used to treat human immunodeficiency virus, or HIV.

Do statins damage mitochondria?

Emerging evidence suggest that statins impair mitochondria, which is demonstrated by abnormal mitochondrial morphology, decreased oxidative phosphorylation capacity and yield, decreased mitochondrial membrane potential and activation of intrinsic apoptotic pathway.

What is FCCP in mitochondria?

FCCP is an protonophore which is widely used to investigate the role of mitochondria in cellular function. FCCP is a potent uncoupler of mitochondrial oxidative phosphorylation. FCCP disrupts ATP synthesis by transporting protons across the mitochondrial inner membrane, interfering with the proton gradient.

What is basal respiration mitochondria?

Basal mitochondrial respiration is determined by monitoring OCR in the absence of any inhibitors. In isolated mitochondria, the basal respiratory measurements (obtained without ADP) are referred to as state II respiration. The difference between maximal and basal OCR is considered to be the spare (or reserve) capacity.

Can mitochondrial disease be cured?

There are no cures for mitochondrial diseases, but treatment can help reduce symptoms or slow the decline in health. Treatment varies from patient to patient and depends on the specific mitochondrial disease diagnosed and its severity.

What are the neurological side effects of statins?

The most common adverse effects include muscle symptoms, fatigue and cognitive problems. A smaller proportion of patients report peripheral neuropathy—burning, numbness or tingling in their extremities—poor sleep, and greater irritability and aggression.

How do you test for mitochondrial function? Traditionally, mitochondrial function has been assessed with Clark-type electrode probes for measuring oxygen consumption, luminescent ATP assays for quantification of total energy metabolism, and MTT or Alamar Blue for determination of metabolic activity. How is mitochondrial damage measured? A very common approach to address mitochondrial bioenergetic dysfunction is…