How to change stroke volume in HPLC?

PROCEDURE

On the front panel of the 2695, select Develop Methods.
On the Methods page, select Default.
On the bottom of the screen is a tab for stroke volume. Select this option.
Adjust the stroke volume to meet the specific flow range:

How do you calculate injection volume HPLC?

For example, if you are currently injecting 20 µL on a 150 x 4.6 mm column and then switch to a 150 x 3.0 mm column, you could estimate the adjusted volume by multiplying 20 x (1.52)/(2.32). Your new volume should be about 8.5 µL.

What is the mobile phase in HPLC?

Sample carried by a moving gas stream of Helium or Nitrogen. High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase).

How is the composition changer per stroke in HPLC?

5.0 min gradient time @ 1 mL/min Gradient volume = 5120 µL Piston strokes for gradient = 64 For 0-50% gradient the composition changer per stroke is 0.8% 38 s gradient time @ 1 mL/min Gradient volume = 640 µL Piston strokes for gradient = 8 For 0-50% gradient the composition changer per stroke is 6.3%

How big is a sample in a HPLC?

The injectorserves to introduce the liquid sampleinto the flow stream of the mobile phase. Typical sample volumes are 5-to 20-microliters (µL). The injector must also be able to withstand the high pressures of the liquid system.

What should the draw speed be for HPLC?

(normal HPLC eluents and samples dissolved in starting eluent ) • => 10 µL injection volume -> Recommended draw speed: 2 – 3 µL/sec. • => 2 µL injection volume -> Recommended draw speed: 0.2 – 0.3 µL/sec. ●Draw speed has to be adapted in case of samples and eluents with higher viscosity.

What is the difference between retention volume and hold up volume in HPLC?

The retention volume ( or time ) minus the Hold-up volume ( or time ). Note the difference between this and the retention volume ( or time ) The packing used in adsorption chromatography. The process where a chemical entity is accumulated on a surface.

How to change stroke volume in HPLC? PROCEDURE On the front panel of the 2695, select Develop Methods. On the Methods page, select Default. On the bottom of the screen is a tab for stroke volume. Select this option. Adjust the stroke volume to meet the specific flow range: How do you calculate injection volume…