What does MacConkey agar test for quizlet?

What does MacConkey agar test for quizlet?

What is MacConkey Agar used for? It is used to isolate and differentiate members of the Enterobacteriaceae based on the ability to ferment lactose.

How do you do a MacConkey agar test?

Preparation of MacConkey Agar

  1. Suspend 49.53 grams of dehydrated medium in 1000 ml purified/distilled water.
  2. Heat to boiling to dissolve the medium completely.
  3. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
  4. Cool to 45-50°C.
  5. Mix well before pouring into sterile Petri plates.

What is MacConkey agar selective for quizlet?

MacConkey agar is selective for gram-negative organisms and contain indicators to differentiate what? Both because it selectively allows only gram-negative bacteria to grow while differentiating which can ferment lactose. You just studied 8 terms!

Is MacConkey agar selective or differential quizlet?

MacConkey agar is both selective and differential. Selective ingredients: It contains bile salts and the dye crystal violet, which inhibit the growth of gram-positive bacteria and select for gram-negative bacteria.

What is the indicator of MacConkey agar?

Key components of the MacConkey medium include crystal violet dye, bile salts, lactose, and neutral red (pH indicator). Crystal violet dye and bile salts halt the growth of gram-positive bacteria. This allows only gram-negative species to form colonies on MAC agar.

What grows MacConkey agar?

MacConkey agar is a selective and differentiating agar that only grows gram-negative bacterial species; it can further differentiate the gram-negative organisms based on their lactose metabolism.

What is the formula for MacConkey agar?

Item No. NCM0017A

Specifications
Brand NEOGEN® Culture Media
Formula Formula Liter Enzymatic Digest of Gelatin 17.0 g/L Enzymatic Digest of Casein 1.5 g/L Enzymatic Digest of Animal Tissue 1.5 g/L Lactose 10.0 g/L Bile Salts Mixture 1.5 g/L Sodium Chloride 5.0 g/L Neutral Red 0.03 g/L Crystal Violet 0.001 g/L Agar 13.5 g/L

What kind of media is MacConkey agar?

MacConkey agar is an example of a medium that is both differential and selective. The presence of bile salts, as well as crystal violet, within the media prevent gram-positive organisms from growing.

Is MacConkey agar defined?

A differential and selective growth medium used to isolate and identify gram-negative bacilli, often enteric pathogens, based on fermentation or lack of fermentation of a sugar added to the media.

What does MacConkey agar test for quizlet? What is MacConkey Agar used for? It is used to isolate and differentiate members of the Enterobacteriaceae based on the ability to ferment lactose. How do you do a MacConkey agar test? Preparation of MacConkey Agar Suspend 49.53 grams of dehydrated medium in 1000 ml purified/distilled water. Heat…