What does the BCA assay measure?
What does the BCA assay measure?
The BCA protein assay is used to quantify total protein in a biological sample. BCA stands for Bicinchoninic acid, which is the key reagent used to produce a colored product. The purple colored product is analyzed in reference to a standard curve in order to quantify protein concentration.
What is the Pierce BCA protein assay based on?
The Thermo Scientific™ Pierce™ BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein.
Is BCA assay light sensitive?
BCA is sensitive and has a broad dynamic range; capable of measuring protein concentrations of 0.5 μg/mL to 1.5 mg/mL. Substances that reduce copper will also produce colour in the BCA assay, thus interfering with the accuracy of the protein quantitation.
What is the BCA microplate assay working range?
Pipette 25 µl of each standard or unknown sample replicate into a microplate well (working range = 20-2,000 µg/ml). Note: If sample size is limited, 10 µl of each unknown sample and standard can be used (sample to WR ratio = 1:20). However, the working range of the assay in this case will be limited to 125-2,000 µg/ml.
How long does a BCA assay take?
Choose the right BCA protein assay for your sample
|Rapid Gold BCA Protein Assay||BCA Protein Assay|
|Incubation time and temperature||5 min at RT||30 min at 37°C|
|Total assay time||50 min||100 min|
|Absorbance||480 nm||562 nm|
What is a BSA standard curve?
A standard curve is a plot of absorbance vs. a varying amount of some known concentration of protein. As long as the volume of the standard samples and the unknown samples are the same the final concentration of the unknown is directly calculated from the least squares line of the standard curve.
How long does it take to do a BCA assay?
BCA Standard Protocol assay was performed on the Modulus using the Absorbance Module and the 560 nm filter. Following a 37°C incubation for 30 minutes, the samples cooled to room temperature before analysis on the Modulus.
Is RIPA buffer compatible with BCA assay?
RIPA Buffer is compatible with the Thermo Scientific™ Pierce™ BCA Protein Assay Kit (Cat. No. 23225). Note: If desired, add protease and phosphatase inhibitors to the RIPA Buffer immediately before use.
What is Micro BCA assay?
The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. The result is an assay for accurately measuring 0.5 to 20 µg/mL protein solutions.
How does the Pierce BCA protein assay kit work?
The Thermo Scientific Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure (A562nm) total protein concentration compared to a protein standard. Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination…
Which is better Pierce or Bradford BCA assay?
Pierce BCA Protein Assays have a unique advantage over the Coomassie dye–based assays (Bradford)–they are compatible with samples that contain up to 5% surfactants (detergents), and are affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
What is the wavelength of the BCA protein assay?
At what wavelength is the BCA Protein Assay measured? The maximum absorbance of the BCA-Cu+1 complex occurs at 562 nm. If a spectrophotometer or plate reader with a 562 nm filter is not available, the purple color may be measured at any wavelength between 540 nm and 590 nm.
Which is the best Thermo Scientific Pierce Protein Assay?
BCA protein assay absorbance spectra. Scans of eight BSA standards (0 to 2000 µg/mL) tested with the Thermo Scientific Pierce BCA Protein Assay Kit (Part No. 23225). The thick line is the 2000 µg/mL sample. The optimum measurement wavelength for this assay is 562 nm.
What does the BCA assay measure? The BCA protein assay is used to quantify total protein in a biological sample. BCA stands for Bicinchoninic acid, which is the key reagent used to produce a colored product. The purple colored product is analyzed in reference to a standard curve in order to quantify protein concentration. What…