What is a CRE dependent virus?
What is a CRE dependent virus?
The first Cre-dependent tracing virus was a PRV that expressed a gene essential for virus replication in neurons, thymidine kinase (TK), in a Cre-dependent manner (DeFalco et al., 2001). A theoretical example is as follows. (1) The virus is first injected into a region of interest with cells expressing Cre.
What is AAV cre?
loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cultured cells or experimental animals.
What is TVA rabies?
TVA is an avian receptor protein that confers infection capability to rabies virus pseudotyped with the avian sarcoma leucosis virus glycoprotein EnvA (red). EnvA-pseudotyped rabies virus is incapable of infecting mammalian neurons in the absence of TVA, restricting rabies virus infection to TVA-expressing cells.
How does CRE loxP work?
How does it work? As alluded to above, the Cre recombinase catalyzes the site specific recombination event between two loxP sites, which can be located either on the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized and bound by a Cre protein, forming a dimer.
What type of virus causes rabies?
Rabies virus belongs to the order Mononegavirales, viruses with a nonsegmented, negative-stranded RNA genomes. Within this group, viruses with a distinct “bullet” shape are classified in the Rhabdoviridae family, which includes at least three genera of animal viruses, Lyssavirus, Ephemerovirus, and Vesiculovirus.
How is Cre recombinase expression dependent on the promoter?
Expression of Cre recombinase from early transgenes was dependent on the characteristics of the genetic promoter used to drive expansion.
Where does CRE dependent selection yield widespread AAV variants?
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA. 2Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, California, USA.
How is Cre recombinase activated by tamoxifen?
Activation of Cre recombinase and selectable gene elimination by 4-hydroxy tamoxifen. The Cre recombinase gene expression may be under the control of the Tet-On system, itself under the control of a cell-specific promoter. The fusion protein Cre recombinase-mutated estrogen receptor is active only in the presence of 4-hydroxy tamoxifen.
How did the CRE bacteria get its name?
CRE got their name from the fact that they are resistant to carbapenems. Carbapenems are an advanced class of antibiotics. They were created in the 1980s to help treat bacteria that could not be treated with other antibiotics.
What is a CRE dependent virus? The first Cre-dependent tracing virus was a PRV that expressed a gene essential for virus replication in neurons, thymidine kinase (TK), in a Cre-dependent manner (DeFalco et al., 2001). A theoretical example is as follows. (1) The virus is first injected into a region of interest with cells expressing…