What is EDC reagent?
What is EDC reagent?
1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, EDAC or EDCI) is a water-soluble carbodiimide usually handled as the hydrochloride. It is typically employed in the 4.0-6.0 pH range. It is generally used as a carboxyl activating agent for the coupling of primary amines to yield amide bonds.
What is EDC and NHS?
EDC, in conjunction with NHS allows, for 2-step coupling of two proteins without affecting the carboxyls of the second protein. First, EDC activates carboxyl groups and forms an amine reactive O-acylisourea intermediate that spontaneously reacts with primary amines to form an amide bond and an isourea by-product.
Is EDC a carbodiimide?
EDC (also called EDAC) is 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride, MW 191.70.
What is EDC HCL?
Alternate Names: EDC hydrochloride is also known as (3-Dimethylamino-propyl)-ethyl-carbodiimide Hydrochloride. Application: EDC hydrochloride is a water soluble carboxyl activating carbodiimide reagent, used for amide bond formation. CAS Number: 25952-53-8. Purity: ≥98% Molecular Weight: 191.70.
What does EDC stand for guns?
Every Day Carry
EDC – Every Day Carry.
What kind of reagent is used for crosslinking?
Crosslinking reagents contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. The availability of several chemical groups in proteins and peptides make them targets for conjugation and for study using crosslinking methods.
Which is more efficient EDC crosslinking or conjugation?
When they are peptides or proteins, these molecules are tens-to-thousands of times larger than the crosslinker and conjugation arms diagrammed in the reaction. EDC crosslinking is most efficient in acidic (pH 4.5) conditions and must be performed in buffers devoid of extraneous carboxyls and amines.
How does Thermo Scientific Pierce EDC crosslinker work?
Learn more Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product.
How does EDC react with a carboxyl group?
EDC reacts with a carboxyl to form an amine-reactive O -acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N -hydroxysulfosuccinimide (Sulfo-NHS), EDC can be used to convert carboxyl groups to amine-reactive Sulfo-NHS esters.
What is EDC reagent? 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, EDAC or EDCI) is a water-soluble carbodiimide usually handled as the hydrochloride. It is typically employed in the 4.0-6.0 pH range. It is generally used as a carboxyl activating agent for the coupling of primary amines to yield amide bonds. What is EDC and NHS? EDC, in conjunction with…