What is Elisa test for hepatitis B?

A solid-phase enzyme-linked immunosorbent assay (ELISA), based on the “sandwich” principle with use of microtiter plates, was developed for the detection of hepatitis B surface antigen (HBSAg). Results could be read within one day by the naked eye or by colorimeter.

What is HBsAg ELISA reactive?

Hepatitis B Blood Tests HBsAg (Hepatitis B surface antigen) – A “positive” or “reactive” HBsAg test result means that the person is infected with hepatitis B. This test can detect the actual presence of the hepatitis B virus (called the “surface antigen”) in your blood.

What did our Elisa test check for?

ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood. An antibody is a protein produced by the body’s immune system when it detects harmful substances, called antigens.

Is ELISA test accurate for hepatitis B?

Tests were processed upon three different antigen–antibody reactions (HBsAg, HBcAb and HBsAb) and virus DNA. The data obtained in this study clearly confirm the existence of HBV in 9.29% of cases by molecular detection (DNA), while 9.02% cases were positive to HBV on ELISA detection (Table 2). Table 2.

Which ELISA technique is used to detect antibodies?

The procedure for a sandwich ELISA involves coating a polystyrene plate with a capture antibody. The analyte or sample is then added, followed by a detection antibody. The detection antibody can be enzyme conjugated, in which case this is referred to as a direct sandwich ELISA.

How many types of ELISA are there?

four
There are four main types of ELISA: direct ELISA, indirect ELISA, sandwich ELISA and competitive ELISA. Each has unique advantages, disadvantages and suitability.

What are the steps in ELISA?

ELISA Step-by-step

Antibody coating. Specific capture antibody is immobilized on high protein-binding plates by overnight incubation.
Protein capture.
Detection antibody.
Streptavidin-enzyme conjugate.
Addition of substrate.
Analysis.

What is Elisa test for hepatitis B? A solid-phase enzyme-linked immunosorbent assay (ELISA), based on the “sandwich” principle with use of microtiter plates, was developed for the detection of hepatitis B surface antigen (HBSAg). Results could be read within one day by the naked eye or by colorimeter. What is HBsAg ELISA reactive? Hepatitis B…