What is Nile red staining?

What is Nile red staining?

Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red stains intracellular lipid droplets yellow. Nile red has also been used as part of a sensitive detection process for microplastics in bottled water.

How to dissolve Nile Red?

Nile red dye (0.01 g) was dissolved in 40 mL of acetone to produce a Nile red stock solution (0.25 mg/mL).

Does Nile Red dissolve in water?

Nile Red (compound A) fluoresces at about 530 nm with good quantum yields in apolar solvents. In more polar ones its fluorescence emission shows a dramatic, and potentially useful, shift to about 640 nm, but its quantum yield is significantly reduced. Further, Nile Red has a very poor solubility in aqueous media.

What is the difference between Nile red and Nile blue?

Structures of Nile red and Nile blue. Nile red has no formal charges and is intensely fluorescent with a high quantum yield in apolar media. Nile blue is a cationic dye and is thus more readily soluble in water than Nile red. It is highly sensitive to pH changes making it a useful pH probe.

What is the purpose of Nile red?

Description. Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells.

Is Nile red toxic?

TOXICITY AND IRRITATION NILE RED: No significant acute toxicological data identified in literature search. All waste must be handled in accordance with local, state and federal regulations. In some areas, certain wastes must be tracked.

How does nile red stain work?

In the assay, Nile Red dye is added to the cell samples and the dye selectively stains lipid droplets within the cells. The dye is minimally fluorescent in solution in water so it is not necessary to wash the cells or remove the dye before analysis.

What is Nile red dye used for?

Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells.

What does Nile blue stain?

Nile blue (or Nile blue A) is a stain used in biology and histology. It may be used with live or fixed cells, and imparts a blue colour to cell nuclei. It may also be used in conjunction with fluorescence microscopy to stain for the presence of polyhydroxybutyrate granules in prokaryotic or eukaryotic cells.

How do you make a Blue Nile?

(Method of Ostle and Holt, 1982) Prepare a 1% aqueous solution of the oxazine dye Nile blue A. Use mild heating to fully dissolve the dye if necessary. Filter the solution before use. Heat-fix smears of the cells and stain with the Nile blue A solution at 55°C for 10 min in a staining jar.

How long does Nile red last?

Optimization of staining parameters with Nile red and BODIPY 505/515. Dye stock solutions should be stored at controlled temperature and protected from the light, for up to 30 days at −20°C, avoiding thus fluorescence loss and evaporation.

What does Nile Red do for a living?

residing in Montreal, Quebec, Canada. He runs the NileRed YouTube channel and social media accounts.

What kind of stain is Nile red used for?

Nile Red is a phenoxazone dye. It is an important stain, which is used to detect intracellular lipid droplets and proteins. Nile Red detects lysosomes and lysosome-related organelles, like gut granules in C. elegans intestinal cells. It exhibits strong fluorescence enhancement and is used for staining SDS (sodium dodecyl sulphate) gels.

How is Nile red used to localize lipids?

Description. Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments (excitation/emission maxima ~552/636 nm in methanol).

What is the excitation maxima of Nile red?

Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments (excitation/emission maxima ~552/636 nm in methanol). For Research Use Only.

What is Nile red staining? Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red stains intracellular lipid droplets yellow. Nile red has also been used as part of a sensitive detection process for microplastics in bottled water. How to dissolve Nile Red? Nile red dye (0.01 g) was dissolved in…