What is the highest DNA band size in 1 kb DNA ladder?

1 kb plus DNA ladders usually have about 14 purified DNA fragments with a reference band at 1000 and 3000 bp for easy orientation. Most 1 kb plus DNA ladders are provided with loading dye which allows a convenient detection of sharp bands after electrophoresis.

What is a 1 kb DNA ladder?

1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 – 10,000 base pairs (bp). This DNA ladder comes pre-mixed with DNA Loading Dye, which is also available for individual sale (Catalog #79018), for loading DNA samples into agarose gel wells.

What is the DNA size standard for?

Preparing the DNA for electrophoresis A DNA marker (also known as a size standard or a DNA ladder) is loaded into the first well of the gel. The fragments in the marker are of a known length so can be used to help approximate the size of the fragments in the samples.

What is a 1 kb ladder?

1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 – 10,000 base pairs (bp). The ladder consists of 13 double-stranded, blunt-end fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1).

What does 1 KB plus DNA ladder do?

Description Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.

How much DNA ladder to use in Neb?

We recommend loading 0.5 μg of 1 kb DNA Ladder diluted in sample buffer. All fragments have a 4-base, 5´ overhangs that can be end labeled using T4 Polynucleotide Kinase (#M0201) or filled-in using DNA Polymerase I, Klenow Fragment (#M0210) (1). Use α- [ 32 P] dATP or α- [ 32 P] dTTP for the fill-in reaction.

How much DNA ladder can you use in one gel?

NEB also offers a Quick-Load version of this ladder with purple dye. Recommended gel percentage range: 0.8-1.2%. Optimum separation on 1%. 1 kb DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel. Mass values are for 0.5 µg/gel lane.

How big is a Takara DNA weight marker?

Takara offers a variety of DNA ladders suitable for different applications. This marker consists of 10 double-stranded DNA fragments ranging from 100 bp to 1 kb in 100-bp units, plus an additional 1.5 kb fragment.

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What is the highest DNA band size in 1 kb DNA ladder? 1 kb plus DNA ladders usually have about 14 purified DNA fragments with a reference band at 1000 and 3000 bp for easy orientation. Most 1 kb plus DNA ladders are provided with loading dye which allows a convenient detection of sharp bands…