What is the purpose of bicinchoninic acid?

Bicinchoninic acid is used to analyze to monitor cuprous ion presence due to its purple coloration via a biuret reactionIn this assay, two molecules of bicinchoninic acid chelate a single Cu+ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.

What does BCA stand for?

BCA

Acronym	Definition
BCA	Bachelor of Computer Applications
BCA	British Crystallographic Association
BCA	Black Contractors Association (various locations)
BCA	Business Cooperation Agreement (various companies)

How do you dilute a BCA sample?

This is how I typically dilute my samples:

Transfer 20 µL of each sample to a new cDNA tube/Eppendorf tubes.
Add 60 µL of deionized water to the samples (dilute by a factor of 4).

How accurate is BCA assay?

Nothing is wrong with BCA assay. It is one of those sensitive assay method for protein estimation. However, Beta mercapto ethanol will send your head spinning when you will do the assay in its prtesence. In fact it interferes with even Lowry’s method too.

How is the bicinchoninic acid ( BCA ) assay used?

The bicinchoninic acid (BCA) assay for protein quantitation The bicinchoninic acid (BCA) assay for protein quantitation The bicinchoninic acid (BCA) assay for protein quantitation The bicinchoninic acid (BCA) assay for protein quantitation

Who is the inventor of the BCA assay?

Bicinchoninic acid assay. From Wikipedia, the free encyclopedia. Jump to navigation Jump to search. BCA protein assay in a 96 well plate. The bicinchoninic acid assay ( BCA assay ), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, now part of Thermo Fisher Scientific,

Is the BCA protein assay sensitive to cuprous cation?

The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid.

Is the Lowry protein assay similar to the bicinchoninic acid assay?

The principle of this technique is quite similar to the Lowry protein assay, which is one of the alternative protein quantification methods.

What is the purpose of bicinchoninic acid? Bicinchoninic acid is used to analyze to monitor cuprous ion presence due to its purple coloration via a biuret reactionIn this assay, two molecules of bicinchoninic acid chelate a single Cu+ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm. What does BCA stand…